© 1971 by The Society for Integrative and Comparative Biology
Effects of Hydrostatic Pressure on Catalysis by Epaxial Muscle Phosphofructokinase from an Abyssal Fish
Department of Zoology, University of British Columbia Vancouver 8, B. C, Canada
SYNOPSIS: Phosphofructokinase (PFK) extracted from muscle of abyssal Coryphaenoides fishes common in the deep waters around the Galápagos Archipelago is extremely unstable upon decompression and extraction and can be recovered only in low activities. Preliminary studies indicate that the pressure responses of the enzyme are complex: At low pressures, the enzyme is activated; at moderate pressures, activity passes through a pressure optimum; at high pressures, maximum catalytic activity is decelerated. At low pressures, the volume change of activation is about 11 cm3/mole at 3°C and increases to about 46 cm3/mole at 28°C. The homologous enzyme from a surface species (Oligoplites mundus) appears to be more pressure sensitive at low temperatures.