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American Zoologist 1974 14(2):543-550; doi:10.1093/icb/14.2.543
© 1974 by The Society for Integrative and Comparative Biology
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Activity of Hydra Cells in vitro and in Regenerating Cell Reaggregates

HANS R. BODE
Max-Planck-Institut für Virusforschung 74 Tübingen, West Germany and Univeristy of California Irvine, California 92664

Hydra were disaggregated into single cells and maintained in a complex medium for a few days. The cells are capable of macromolecular synthesis, of processing RNA, and of carrying out the formation of a nematocyst capsule. Single cells can be centrifuged into aggregates which will develop into normal hydra. Changes in morphology and cell composition are described. Interstitial cells after a 1-day delay resume their normal mitotic and differentiation activity in the aggregates. The method provides new means for studying problems of cell differentiation in hydra.


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