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American Zoologist 1975 15(1):93-106; doi:10.1093/icb/15.1.93
© 1975 by The Society for Integrative and Comparative Biology
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Ontogeny, Phytogeny, and Cellular Cooperation

LAURENS N. RUBEN
Department of Biology Reed College, Portland, Oregon 97202

The capacity of adult newt (Triturus viridescens) spleen cells to secrete antibody at 4 C allows simultaneous visualization and quantification of non-secretory (S–) and secretory (S+) rosette forming cells (RFC). Visualization of mammalian S+ RFC requires 37 C, a temperature at which S– RFC appear to be fragile. RFC can be distinguished as S– or S+ due to whether one or more layers of erythrocytes adhere to the surface of sensitized spleen cells. Different doses of horse erythrocytes (HRBC) affect newt S– RFC and S+ RFC differentially. By varying the time between injections of different concentrations of chicken erythrocytes (CRBC, the "carrier") and a constant dosage of CRBC-TNP (trinitrophenyl, the hapten) it is possible to measure "helper" activity that correlates with the population of S– RFC and is both dose and time dependent. By varying assay time after "helper" activity has been maximized, one can determine the cytodynamics of anti-TNP antibody producing cell (APC) activity. For the first time these morphologically separable RFC can be related to their suspected physiologic behavior. A shift from S– RFC to S+ RFC takes place during the immune response. That similar dose-dependent response curves can be shown in adultRana pipiens suggests that the newt responses represent a fundamental vertebrate pattern.


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