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American Zoologist 1976 16(2):125-130; doi:10.1093/icb/16.2.125
© 1976 by The Society for Integrative and Comparative Biology
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INSECT NEUROSECRETION IN VITRO

E. P. MARKS and G. M. HOLMAN
Metabolism and Radiation Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture Fargo, North Dakota 58102
Veterinary Toxicology and Entomology Laboratory, Agricultural Research Service, U.S. Department of Agriculture College Station, Texas 77840

The use of cell and organ culture for the study of insect neurosecretion is a relatively new methodology that is developing rapidly. Recent studies indicate that although it is possible to correlate the presence of stainable neurosecretory material and electrondense neurosecretory granules with the presence of neurohormones in some systems, the actual release of the hormone can be detected only by bioassay. Bioassay systems for both tropic hormones and peptide metabolic hormones have been used to demonstrate the synthesis, storage, and release of neurohormones by cultured neuroendocrine organs. The experimental flexibility permitted by in vitro techniques is rapidly making it the method of choice in neuroendocrinological studies.


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