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American Zoologist 1991 31(3):580-591; doi:10.1093/icb/31.3.580
© 1991 by The Society for Integrative and Comparative Biology
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Reagents Specific for MHC Class I Antigens of Xenopus1

MARTIN F. FLAJNIK, ELEANOR TAYLOR, CAMILO CANEL, DARIO GROSSBERGER and LOUIS DU PASQUIER
Department of Microbiology and Immunology, University of Miami Miami, Florida 33101
Department of Structural Biology, Stanford University, Stanford California 94035
Basel Institute for Immunology Grenzacherstrasse 487, ostfach 4005 Basel, Switzerland

The preparation of antisera and monoclonal antibodies specific for frog MHC class I molecules is presented. Alloantisera specific for the native and denatured forms of Xenopus MHC class I proteins have been obtained, and have been used to determine, by Western blotting, the MHC haplotypes of animals derived from Fl crosses or backcrosses. The alloantisera have also been used to partially purify class I molecules for immunizations of mice in order to produce monoclonal antibodies (mAbs) and xenoantisera. The mAbs recognize polymorphic determinants of Xenopus class I molecules; all are specific for the denatured and cytoplasmic forms of class I. One of the mAbs also recognizes the cell surface form of class I, but none recognize class I alpha chains associated with beta2-microglobulin under "native" conditions. The different antibodies help to define different forms of the class I molecules expressed by different tissues


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