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Integrative and Comparative Biology Advance Access originally published online on February 16, 2006
Integrative and Comparative Biology 2006 46(2):207-214; doi:10.1093/icb/icj015
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© The Society for Integrative and Comparative Biology 2006. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta)

Angela V. Klaus1,* and Valerie Schawaroch{dagger},§
*Microscopy and Imaging Facility, American Museum of Natural History Central Park West at 79th Street, New York, NY 10024-5192, USA
{dagger}Division of Invertebrate Zoology, American Museum of Natural History Central Park West at 79th Street, New York, NY 10024-5192, USA
§Department of Natural Sciences, Baruch College 1 Bernard Baruch Way, Box A-0506, New York, NY 10010-5585, USA

Correspondence: 1E-mail: avklaus{at}amnh.org

The use of confocal laser scanning microscopy (CLSM) for imaging arthropod structures has the potential to profoundly impact the systematics of this group. Three-dimensional visualization of CLSM data provides high-fidelity, detailed images of minuscule structures unobtainable by traditional methods (for example, hand illustration, bright-field light microscopy, scanning electron microscopy). A CLSM data set consists of a stack of 2-D images ("optical slices") collected from a transparent, fluorescent specimen of suitable thickness. Small arthropod structures are particularly well suited for CLSM imaging owing to the autofluorescent nature of their tissues. Here, we document the practical aspects of a methodology developed for obtaining image stacks via CLSM from autofluorescent insect cuticular structures.


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